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A non-natural amino acid containing the hydroquinone group was synthesized and shown to incorporate at selective positions within a peptide by using standard solid phase peptide synthesis.
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Solid S1 was characterized using standard solid state procedures.
The tetrapeptide KWKK was synthesized using standard solid phase peptide synthesis methods employing Fmoc protecting groups and purified by reverse-phase HPLC.
Messenger RNA was purified using poly(A)+ mRNA selection, and sequenced using standard SOLiD RNA-seq procedures.
The Cbp peptide (ISAMpYSSVNK) was synthesized by GenScript (Piscataway, NJ) using standard solid-phase peptide synthesis methods and then resuspended in purified water before use.
Sequences containing multiple enhancing point variations were synthesized using standard solid-phase synthesis and purified.
Library synthesis was performed using standard solid-phase peptoid synthesis protocols.
The peptide spacer NH2-GFL-OH was synthesized using standard solid-phase Fmoc peptide synthesis.
The standard approach for response evaluation in MBC continues to rely on anatomic imaging and changes in tumor size by using standard criteria such as Response Evaluation Criteria in Solid Tumors (RECIST) and anatomic imaging, mostly CT [ 55, 56].
The hospital microbiology laboratory conducted isolation (both in solid and liquid media), identification, and antimicrobial susceptibility testing by using standard methods (12) on all specimens.
Linear GRGDSC, GRDGS, and cyclic RGDfC were synthesized using standard Fmoc solid phase peptide synthesis protocol.
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