Sentence examples for by using standard cloning from inspiring English sources

Plasmid constructs were generated by using standard cloning techniques and were confirmed by sequencing.

Since TALE repeats can be assembled cheaply by using standard cloning methods in the laboratory (Zhang et al., 2011; Huang et al., 2011), combined with the low cost of maintaining X. tropicalis, our findings suggest that TALENs offers a relatively cheap and efficient means of generating targeted mutations in genes in this system.

These two fragments were then cloned in the sense orientation into the ClaI and the KpnI-sites of pHR-CMV.GFP.W (CMV.SIN) (kind gift from D. Trono, Lausanne, Switzerland) and pHR-EF1α.GFP.W (EF1α.SIN) (kindly provided by N.B. Woods, Lund, Sweden) using standard cloning procedures to generate pHR-2 × 250 bp.CMV.GFP.W.2 × 250 (d2 × 250 bp.CMV) and pHR-2 × 250 bp.EF1α.GFP.W.2 × 250 (d2 × 250 bp.EF1α).

CagA and CagAEPISA (provided by H.Higashi) were cloned into pMT vector using standard cloning procedures.

Plasmids were constructed using standard cloning procedures and confirmed by DNA sequencing.

Four general use plasmids were made using standard cloning techniques and confirmed by restriction enzyme analysis and sequencing.

Httex1 was labeled with mRFP by replacing EGFP in the above vectors with mRFP1 (Q66T) [77] using standard cloning techniques.

Different plasmids were assembled using standard cloning techniques and their sequence was confirmed by DNA sequencing (ABI Model 377).

The plasmid pYES-TagBFP (mito-TagBFP) was obtained by replacing BFP in pYES-BFP (Westermann and Neupert, 2000) with TagBFP using standard cloning techniques.

pCS2+Axin, pCS2+AxinΔDIX, and pCS2+Axin 351-701) (all were Flag-tagged) were cloned using standard cloning procedures.

This fragment was blunt cloned into the pZero-1 (Invitrogen) Eco RV site using standard cloning procedures.