Moreover, we showed, by using specific siRNA against TBK1, that this cellular kinase does not seem to be required for the VZV-dependent phosphorylation of IRF3 since the partial depletion of TBK1 does not impact on the up-shift observed.
To confirm the role of moesin in VEGF-C-induced actin cytoskeleton remodeling, we silenced moesin by using specific siRNA (Fig. 2C: S - Scrambled SiRNA; M - Moesin SiRNA).
To further ascertain the role of autophagy in NDV/FMW-mediated oncolysis in drug-resistant A549 cells, we knocked down expression of ATG5, which is involved in autophagosome formation, in drug-resistant A549 and parent cells by using specific siRNA targeting ATG5, and analyzed NDV/FMW-induced cell death by MTT assay.
CHIP, BAG-1 and HspBP1, respectively, were depleted in mouse BMDCs using specific siRNAs, followed by infection with a recombinant adenovirus that expresses the immune dominant SIINFEKL peptide of ovalbumin fused to luciferase and GFP (Ad-LOG) [35].
Egr1 or Mkx genes were knocked down using specific siRNAs, which were transfected into tenocytes by the GenMuteTM siRNA Transfection Reagent (SignaGen®).
By selectively downmodulating bfl-1 using specific siRNA we could induce apoptosis in fludarabine-resistant B-CLL cells, showing that bfl-1 has a protective role against apoptosis in these cells.
Recently, we reported that in TNBC cell lines an increase of proliferation by17β-estradiol was dependent on GPR30, as it was completely prevented by knock-down of GPR30 expression using specific siRNA [ 12].
As Smad1 is the positive regulator but GSK3 β is the negative regulator of BMSC osteogenesis, we moved forward to explore which was the major target of miR-26a by knocking down GSK3 β or Smad1 using specific siRNA (Supplementary Figure S3).
