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Every 30 min a procedure checks if new data have been acquired and eventually, if in binary format, they are converted by using specific procedures driven by RTLoader.
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Invert emulsions were fabricated in several experiments using specific procedures.
The immunoreactive fraction of 89Zr-DFO-trastuzumab samples was determined by using specific radioactive cellular-binding assays following modified procedures derived from Lindmo et al.[53] Briefly, BT-474 or MDA-MB-468 cells were suspended in micro-centrifuge tubes at concentrations of 5.0, 4.0, 3.0, 2.0, 2.0, 1.5, and 0.5×106 cells/mL in 500 µL PBS (pH 7.4).
Before testing of study participants, all participating electrophysiologists attended a standardization training meeting, and all centers were certified by submitting three satisfactory complete examinations using specific study procedures by the central reading site (J.C.A. at the Albert Einstein College of Medicine, New York, NY).
To evaluate the effects of protein tyrosine phosphorylation on COX activity, cells were preincubated with the protein kinase inhibitor Tyrphostin 47 (300 µM) for 30 min. Concentration of PGE2 in the cell incubation medium was determined by radioimmunoassay, using specific antibodies according to procedures reported elsewhere (Gobbetti et al., 1999).
Abstracted data for all case infants were reviewed by clinical geneticists using specific classification criteria, including standardized case definitions and confirmatory diagnostic procedures (Rasmussen et al. 2003).
Users can define genomic procedures using specific interfaces.
These were evaluated for the duration of the second period by random weekly observations from the QCN using specific check-list files for each procedure.
In the case of electroporation some specificity can be gained by targeting the plasmids to certain brain regions, by "aiming" the electrical pulse, or by using the procedure at specific developmental time points.
DNA constructions were carried out by using established procedures [31] or following the instructions provided with specific reagents.
Cells were extracted in RIPA buffer, and 100 μg of extract proteins assessed for the expression of specific proteins by using standard immunoblotting procedures, as previously described [ 24].
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