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miR-214mut could be distinguished from miR-214 by using specific probes and primers in qRT-PCR assay.
Therefore, differences between homeologous genes may exist and this problem needs to be addressed by using specific probes or homeologous-specific primers in qRT-PCR analysis.
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Next, their retinal localization was investigated by ISH using specific probes.
Replication intermediates were analysed by Southern blotting using specific probes against the fragment of interest.
The detection was done by PCR amplification, using specific probes on both sides of the alternative splice junction.
Similar results were obtained when the effects of treatment with RNase A on the chromatin structure of specific genomic regions were determined by Southern blotting using specific probes (Figure 4).
ROS/RNS levels were determined by flow cytomtery using specific probes.
> We experimentally verified the presence and absence of miR1511 by Northern blotting using specific probes and U6 snRNA as an internal reference.
The expression of both up- and down-regulated genes in the mutants was analyzed at 24 h and 48 h of growth by northern-blot hybridization using specific probes (Additional file 3: Table S3).
The precise distribution of the GS transcripts in the different cell types of the leaves, stems and roots of poplar was examined by in situ hybridization (ISH) using specific probes.
This assumption was further supported by our in situ hybridization analysis using specific probes against the IFN-α-inducible genes, Ifnal, Ifit, Irf7, and Oas1g, and the plasmacytoid dendritic cell marker Tlr7.
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