Sentence examples for by using specific primer from inspiring English sources

To induce point mutations within Xbp1U was amplified by using specific primer 532G (5′-CCAGCAGGTGCAGGCCCAGTTGTC-3′).

Specimens positive for influenza A by RT-PCR were further tested for subtypes H1, H3, and H5 by using specific primer sets.

GFP fusion constructs of ZmPti1b, -c and -d were cloned similarly by using specific primer pairs which generated BamHI compatible BglII restriction sites.

To test whether HuR would preferentially bind to only a specific caspase-2 transcript, we performed a crosslinking-RNP-IP assay by using specific primer pairs encompassing specific regions of the different splice variants (roman numerals in Figure 1b).

The methylation status of BRMS1 gene in tissue samples was detected by conventional MSP by using specific primer pairs for both the methylated and unmethylated promoter sequences as previously described (Chimonidou et al, 2013).

After immunoprecipitation of DNA fragments bearing the histone modifications mentioned above, qPCR was performed by using specific primer pairs (Supplementary Table S1) spanning approximately −200 bp to +50 bp of the Ins1, Ins2 and Pdx1 genes.

Alternatively, amplification of two adjacent rDNA fragments of enterococci was performed by using specific primers.

Rapid diagnosis of Escherichia coli (E. coli), Salmonella and Vibrio cholera pathogens can be done with simultaneously in a single multiplex PCR assay by using specific primers with adjusted PCR conditions.

Specific transcripts were amplified by using specific primers.

PCR amplification was performed by using specific primers (primer sequences available on request).

Presence or absence of 35 T3Es in strains used in this work was achieved by using specific primers for PCR amplification, as well as by dot blot hybridization.