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Immunohistochemistry (IHC) was performed by using specific monoclonal antibody for CD20 (mouse anti-human, DAKO), as previously reported [ 31].
Immunohistochemistry was performed by using specific monoclonal antibodies for EGFR Abcamm Ltd, ab2430) based on a standardized protocol.
Nasopharyngeal aspirates were tested by indirect immunofluorescence, by using specific monoclonal antibodies (Argene), for influenza viruses A and B, respiratory syncytial virus, parainfluenza viruses 1 3, and adenoviruses.
Recently our studies found that reduced levels of erythrocyte CR1 may reflect disease activity in lupus patients by using specific monoclonal antibody CR1-2B11 [ 14, 15].
For viral isolation, if a cytopathic effect was observed, IFA was performed to identify the virus in infected cell cultures by using specific monoclonal antibodies to H1, H3, and H5.
The triple helical citC1 peptide was synthesized as described in [ 28], analyzed with mass spectrometry, Western blots and crystallographic analysis, and checked by ELISA by using specific monoclonal antibodies detecting either triple helical or denatured forms of the peptide [ 21].
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E-cadherin protein expression was studied by immunohistochemistry using specific monoclonal antibodies against E-cadherin (HEC-D10; Zymed Laboratories, San Francisco, CA, USA).
Antigen retrieval was performed treating sections by proteinase K. Phospho-EGF Receptor (Tyr1173) (EGFRtyr1173) and Phospho-EGF Receptor (Tyr1068 EGFRtyr106868) expression was detected by IHC using specific monoclonal antibodies (Cell Signaling).
Cell surface glycan expression pattern for both models was studied by flow cytometry using specific monoclonal antibodies against Lewis antigens and specific lectins (results shown in Figure 2).
CM were analysed for type I and type III collagen, MMP-1, MMP-2, MMP-9, TIMP-1 and TIMP-2 by Western blotting using specific monoclonal antibodies.
Nm incubation with curcumin for 3 h had no effect on the levels of Opa, Opc or pilin expression as assessed by quantitative immunoblotting using specific monoclonal antibodies (data not shown).
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