Triglycerides, total cholesterol and free fatty acids were assayed by colorimetry using specific commercial kits (Triglyceride/Free glycerol reagent from Sigma, Cholesterol CHOD-PAP from Roche Diagnostic, NEFA from Randox, respectively), and phospholipids were assayed by phosphorus determination [22].
All hormones of the present study (salivary cortisol, serum cortisol, and serum ACTH) were analyzed by specific electrochemiluminescence assays using specific commercial kits at a laboratory, whereas serum glucose levels were analyzed by an enzymatic assay of hexokinase.
Cytokine mRNA of extracts from mouse nervous tissues, collected at different days a.i., were quantified by real-time RT-PCR using specific commercial kits (Bionner, CA, USA).
The statistical analysis of results was addressed by use of both simple linear models and more complicated quadratic models using specific commercial software.
Myeloperoxidase was also measured using specific commercial ELISA (Assay Designs, Ann Arbor, MI).
All hormones were assayed in duplicate using specific commercial RIA kits, except for catecholamines (high-performance liquid chromatography).
AsV was detected by standard RT-PCR (7 ), coupled with hybridization by using specific biotin-labeled probes with the commercial Kit Hybridowell universal (Argene, Varilhes, France).
These four purification methods were prepared by using specific ligands (RAC and RAC-ovalbumin) and commercial ligands (protein G and protein A), respectively.
The presence of GPB Ss antigens on the surface of the RBCs was detected by the hemagglutination test using specific gel cards (Diamed AG, Morat, Switzerland) and appropriate commercial antibodies.
Commercial immunoprecipitation reagent using specific antibodies directed against HDL particles could be effective in serum with elevated triglycerides [33].
