Land cover products based on remote sensing imagery can indeed significantly be improved by using smarter algorithms, better timing of image acquisition, improved class definitions.
In these experiments, total RNA was purified by oligo (dT) beads and converted to cDNA for PCR amplification using SMARTer PCR cDNA Synthesis Kit (Clontech).
However, the abovementioned 3′ skew bias has been faced in different approaches, like the cDNA transcription using SMARTer [ 28] or the additional mRNA enrichment by its 5′cap [ 38, 39].
Briefly, the methodology involves cDNA synthesis and amplification using SMARTer cDNA synthesis kit (TaKaRa BIO/Clontech, Mountain View, CA), normalization using Trimmer kit (Evrogen, Moscow, Russian Federation), fragmentation by sonication, end-polishing, ligation of adaptors, and amplification of the 454-ready sample.
RACE ready cDNAs were generated using SMARTer RACE cDNA synthesis kit (Clontech).
For all samples, double-stranded cDNAs were synthesized from 1 μg of total RNA using SMARTer PCR cDNA synthesis kits (Clontech, Palo Alto, CA).
Then RACE Ready first-strand cDNA was synthesized using SMARTer™ RACE cDNA Amplification Kit (Clontech) according to the user's manual.
We can start by keeping pollution out of the lakes to begin with by using the smarter green infrastructure tools that the City of Chicago pioneered.
The gut cDNA was prepared by using a SMARTer™ PCR cDNA Synthesis Kit (Clontech, USA) and an Advantage 2 PCR Kit (Clontech, USA) as described previously [ 38].
The full-length bach1b cDNA sequence was isolated by using the SMARTer™ RACE cDNA Amplification Kit, according to the manufacturer's instructions (Clontech) and deposited in NCBI (accession number KJ420533).
