Sentence examples for by using small interfering from inspiring English sources

N-Terminal coiled-coils are also required for GCC185 function as determined by plasmid rescue after GCC185 depletion by using small interfering RNA in cultured cells.

To confirm the role of NF-kappaB in the induction of Cox-2 by p53, we inhibited NF-kappaB in HCT116-p53ind cells by using small interfering (si) RNA (Figure 2d).

By using small interfering RNA, or siRNA, scientists have knocked out a specific gene's expression and then looked at the result.

The specificity of the mutant protein bands was instead confirmed by using small interfering RNA (siRNA) to perform gene specific knockdowns of the mutant CREBBP and EP300 proteins.

We then confirmed the independence of VSV growth on cell-cycle phase by using small interfering RNA (siRNA) to specifically target cyclin D1 and cyclin-dependant kinases (CDK) 4. Ablation or reduction of the expression of these cell cycle components did not result in attenuation of VSV replication in any cell line tested but significantly arrested cells in G1.

Caspases are pointed out to contribute to the regulation of cell proliferation [10], [11], [15], and we have recently reported that caspase-7 has a novel function in the progression of mitosis by using small interfering RNAs (siRNAs) and short hairpin RNA (shRNA) directed towards caspase-7 [22].

Western blotting with an antibody to PTEN demonstrated that PTEN protein expression is knocked down by >50% using small interfering RNA against PTEN (Fig. 2A and B).

The effect of S100A6 on resistance to TNF-α by inhibition of the p53 caspase 8–caspathwaypathway of MLL AFF1-positive ALL cell lines were also confirMLL AFF1-positivesing smALL interfering RNA against S100A6.

To further confirm that PIK3CA can play a role in transformation induced by mutant K-ras, we blocked endogenous PIK3CA expression in cultured OSE cells transfected with mutant K-ras by RNA interference using small interfering RNAs (siRNA).

In this study, we explored the biological roles of TPM3 in hepatocarcinogenesis and involved molecular mechanisms by TPM3 knockdown using small interfering RNA (siRNA) in human HCC cell lines.

Table  2 summarizes the tissue-specific pro-proliferative and anti-apoptotic functions of PXR; in these studies, the activity of PXR was enhanced by PXR overexpression or pharmacologic activation through the use of PXR ligands, or reduced by PXR knockdown using small interfering RNA.