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The level of miRNA expression was calculated from a standard curve constructed by using small RNAs from CAPAN-1 cells.
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We further confirmed this finding by using small RNA sequencing of bone marrow RNA from 8-day-old mice.
Indeed recent studies have identified that Botrytis cinerea suppresses host immunity by hijacking host RNA interference pathways using small RNAs [ 66].
Such end polymorphism of miRNAs has also been observed by others using small RNA cloning approach [43], [65] and other methods, such as RNA-primed Array-based Klenow Extension (RAKE) [31].
Caspases are pointed out to contribute to the regulation of cell proliferation [10], [11], [15], and we have recently reported that caspase-7 has a novel function in the progression of mitosis by using small interfering RNAs (siRNAs) and short hairpin RNA (shRNA) directed towards caspase-7 [22].
RNA interference was performed by using small interfering RNAs purchased from Dharmacon, USA.
We therefore investigated the contribution of each UPR branch to CHOP gene induction by using small interfering RNAs (siRNAs) targeting the corresponding UPR transcription factors.
To study the function of ACSVL3, we performed loss-of-function by using small interfering RNAs to target ACSVL3 and examined stem cell marker expression, neurosphere formation and tumor initiation properties.
By using small interfering RNA, or siRNA, scientists have knocked out a specific gene's expression and then looked at the result.
We then confirmed the independence of VSV growth on cell-cycle phase by using small interfering RNA (siRNA) to specifically target cyclin D1 and cyclin-dependant kinases (CDK) 4. Ablation or reduction of the expression of these cell cycle components did not result in attenuation of VSV replication in any cell line tested but significantly arrested cells in G1.
This was corroborated by using small interfering RNA against Smurf2.
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