By using single-molecule electrophysiology, we noted that a commonality of various truncation FhuA protein pores was the occurrence of 33% blockades of the unitary current at very high transmembrane potentials.
We followed events during loading of an E. coli β clamp in real time by using single-molecule FRET (smFRET).
We then sequenced the entire plasmid in pYHCC by using single-molecule real-time sequencing (Pacific Biosciences, Menlo Park, CA, USA) as described (8 ).
Jennie Flint (University of Cambridge, UK) presented ongoing work on the detection and characterization of Aβ aggregates by using single-molecule techniques.
In this study, we visualized the sequential steps during loading of the dimeric Escherichia coli β2 clamp onto a DNA primer template by the γ clamp loader (δγ3δ′) by using single-molecule Förster resonance energy transfer (smFRET).
Recently, by using single-molecule atomic force microscopy (AFM), it was shown that PV-IgG as well as AK 23 directly interfered with homophilic Dsg 3 binding under cell free conditions (Heupel et al. 2007) which supports the hypothesis of direct inhibition of Dsg 3 binding in PV (Stanley and Amagai 2006).
For WGS-based phylogenies, we sequenced to closure the genomes of ST-459 strain NGBS061 and ST-452 strain NGBS572 (GenBank accession nos. CP007631 and CP007632, respectively) by using single-molecule real-time (SMRT) sequencing (Pacific Biosciences, Menlo Park, CA, USA).
Collectively, through our findings we establish an in vivo model of TLR9 binding and activation by CpG DNA using single molecule fluorescence techniques for single cell studies.
In this case, separation of conformers is maximized by the differences in lifetime and anisotropy using single molecule Förster Resonance Energy Transfer (smFRET) data.
This observation has been corroborated by the study of Wnt gene expression using single molecule FISH (smFISH) in C. elegans (Harterink et al., 2011).
One such example is provided by studies on the hepatitis C virus (HCV) NS3 helicase using single molecule mechanical experiments.
