Sentence examples for by using simultaneous staining from inspiring English sources
The expression of pluripotency markers Nanog and Oct4 in these cells was evaluated by using simultaneous staining with appropriate antibodies.
All targets were treated simultaneously by using the simultaneous integrated boost (SIB) technique.
Expression of the SP receptor (NK1) was detected by using immunocytochemical staining and PCR.
Constructs were harvested after 12 weeks and evaluated grossly and microscopically by using histologic stains.
Intracellular staining of Foxp3 was conducted by using the Foxp3/transcription factor staining buffer set, according to the instructions provided by the manufacturer (eBioscience).
Cells were harvested, and early as well as late apoptotic changes were detected by simultaneous staining with Annexin and PI, using an Annexin V FITC staining kit (Sigma, St Quentin Fallavier, France).
Before we adopted the method of dual staining of FoxP3 and the active form of caspase 3, we confirmed that the results of single staining for both proteins were similar to results generated by their simultaneous staining data not shown.
Apoptotic cells were detected by staining of whole splenocytes with annexin V and propidium iodide (BD Pharmingen), accompanied by simultaneous staining with appropriate fluorochrome-conjugated antibodies to CD4, B220, CD19, CD11c, or CD11b.
To examine the intracellular translocation of the nanoparticles, the localization of AgNPs in HePG-2 cells was tracked by simultaneous staining of cell nucleus.
Specificity of the immunostaining was additionally controlled by simultaneous staining of breast cancer samples with known ErbB2, cyclin D1, p53 and Ki67 expression patterns.
