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To identify the cytotoxicity of ursolic acid, we first examined whether ursolic acid could inhibit the proliferation of CRC by using several cell lines including SW480, SW620, LoVo, and RKO.
A recent study by Nagaraja and colleagues [ 39] describes gene expression patterns corresponding to normal breast, noninvasive breast cancer, and invasive breast cancer by using several cell lines.
By using several cell lines we will better be able to identify the genes critical for ES differentiation as these should be common for all three.
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To understand why the adult Apoer2 null cerebellum is reduced in rostrocaudal length, sagittal sections were immunostained by using several Purkinje cell markers (Fig. 2).
To examine whether activation of CREB results in loss of AP-2α expression, we inhibited CREB phosphorylation in A375SM and C8161-c9 cells by using several kinase inhibitors such as H-89 (PKA/MSK pathway), KN-93 (CaMKIV pathway), SL0101-1 (pathwaypandway), and Triciribine (Akt pathway).
Neurons were distinguished from glial cells by using several morphological features including neuron size, shape, and spatial orientation typical for specific layers, sectors, and brain nuclei.
As Timp1 may stimulate cell proliferation by using several distinct signalling pathways, such as MAPK, the inhibitory effect of CLA on this gene may reduce lymphoproliferation.
Terashima et al. reported the importance of a telomerase assay as a possible predictor of the response to anti-cancer chemotherapy by using several human gastric and breast cancer cells [ 26].
In conclusion, to our knowledge, this study is the first to investigate the estrogenic activity of A. pilosa aqueous extract by using several in vitro assays in MCF-7 cells.
This was followed by a more detailed study of several human cancer cell lines including breast and lung cancer cells which metastasized into distant organs in nude mice by using several chemokine GPCRs.
The obligatory role of T cells in lupus pathogenesis has been demonstrated by using several genetically engineered mouse models [ 30].
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