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In another previous study about ethanol tolerance in E. coli [ 15], ethanol-tolerant strains were obtained by using serial transfer culture experiments.
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Adaptation to BL21 in LB broth used serial transfer, as described elsewhere.
Adaptation to IJ1133 in LB broth used serial transfer, as described elsewhere (Heineman and Bull 2007; Bull et al. 2011).
Positive samples were quantified by using serial dilutions.
These values were determined by using serial sections with Hoechst nucleus staining.
A standard curve was generated by using serial dilutions of CaCO3.
Numbers of viable bacteria were estimated by limiting dilution using serial ten-fold dilutions of samples.
The captured image was analyzed by the image processing toolbox in the MATLAB software, to extract weed information in the image, which is then transferred to a microcontroller using serial port interface.
Efficient partition of hybrid meshes has been accomplished by transforming them to suitable graphs and using serial graph partitioning algorithms.
Exact quantification was achieved by serial dilution with cDNA produced from total RNA extracts using serial dilution steps.
All these modulation types are used in serial transfer mode.
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