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These values were determined by using serial sections with Hoechst nucleus staining.
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Human cell incorporation was assessed as the number of human dystrophin-positive myofibers divided by total myofibers per section using serial sections at 100-µm intervals along the entire muscle length.
Immunohistochemical (IHC) analyses were performed using serial sections.
Immunohistochemical experiments using serial sections showed that cfos+-activated neurons also expressed TRPV1 and/or Nav1.8.
We investigate the three-dimensional structure of a model γ γ′ alloy using serial sectioning and digital reconstruction.
Using serial section, intra-hepatic PD1 and PD-L1 expression was also detected dynamically by immunohistology.
* median (range) The SLN were assessed using serial sectioning and immunohistochemistry[ 20, 21].
Quantification of the histological analysis around the ventral vessels based on MFI in dotted circles (middle) and cell number of the accumulated MHC class II+ cells in dotted circles by using serial frozen sections stained with anti-MHC class II antibody and Hoechst (right).
Quantification of the histological analysis around the ventral vessels based on MFI in dotted circles (bottom left) and cell number of the accumulated MHC class II+ cells in dotted circles by using serial frozen sections stained with anti-MHC class II antibody and Hoechst (bottom right).
Quantification of the histological analysis around the ventral vessels based on MFI in dotted circles (left bottom) and cell number of the accumulated MHC class II+ cells in dotted circles by using serial frozen sections stained with anti-MHC class II antibody and Hoechst (right bottom).
Quantification of the histological analysis around the ventral vessels based on MFI in dotted circles (left bottom) and cell number of the accumulated CD4+ cells in dotted circles by using serial frozen sections stained with anti-CD4 antibody and Hoechst (right bottom).
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