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The functional avidity of these effector populations was assessed by using serial peptide concentrations and defining the SD50 as the peptide concentration yielding half-maximal counts in the IFN-γ ELISPOT assay.
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Positive samples were quantified by using serial dilutions.
These values were determined by using serial sections with Hoechst nucleus staining.
A standard curve was generated by using serial dilutions of CaCO3.
The epitope was fine mapped using serial truncations from peptide termini and showed the optimal epitope to be HKHYLVCNYGPSGN (Figure 2C).
Numbers of viable bacteria were estimated by limiting dilution using serial ten-fold dilutions of samples.
Protein estimations were carried out by a micro Bradford assay using serial dilution of BSA to establish a standard curve.
All assays were performed in duplicate and by the standard curve method using serial cDNA dilution.
Exact quantification was achieved by serial dilution with cDNA produced from total RNA extracts using serial dilution steps.
Using serial section, intra-hepatic PD1 and PD-L1 expression was also detected dynamically by immunohistology.
Tumour growth was monitored using serial BLI.
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