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Our approach incorporated a standard curve to allow determination of the relative number of cDNA molecules of a given gene by using serial dilutions of an arbitrary cDNA sample.
After a 30 min incubation period at room temperature, the samples were evaluated spectrophotometrically at 548 nm and OD values – in comparison with a standard curve was determined in culture medium by using serial dilutions of sodium nitrite – represented total stable metabolites of NO.
Positive samples were quantified by using serial dilutions.
A standard curve was generated by using serial dilutions of CaCO3.
The limit of detection was measured by using serial dilutions of a Plasmodium culture and was 0.3 –3 parasites/μL.
Adenovirus genome copy numbers were calculated by using serial dilutions of an adenoviral reference DNA as a standard.
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Firstly, different concentrations of factor IX which comprised of 0, 2320, 3480, 4640, and 5800 nM were prepared by using serial dilution.
All the synthesized compounds, were studied for their in vitro antimicrobial activities, against four bacterial strains and two fungal strains by using serial dilution method.
In this experiment, the different concentrations of NaCl which comprised of 0, 30, 60, 125, 250, and 500 mM were prepared by using serial dilution, and they were added to a constant volume of GNP.
All compounds were evaluated for in vitro antibacterial activities against Escherichia coli and Staphylococcus aureus strains and in vitro antifungal activity against Candida albicans and Aspergillus niger strains by using serial dilution method.
A standard curve was plotted for each primer probe established by using serial dilution of pooled cDNA from tissues.
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