Sentence examples for by using serial cycles from inspiring English sources

This technique is based on the phenomenon that minute amounts of PrPsc in the sample are able to convert cellular prion protein, which is added as substrate, into the disease-associated form and – by using serial cycles of incubation and sonication – is able to amplify PrPsc in quantities that are detectable by rapid postmortem tests.

Positive samples were quantified by using serial dilutions.

These values were determined by using serial sections with Hoechst nucleus staining.

A standard curve was generated by using serial dilutions of CaCO3.

Numbers of viable bacteria were estimated by limiting dilution using serial ten-fold dilutions of samples.

All assays were performed in duplicate and by the standard curve method using serial cDNA dilution.

Samples were heated for 2 min at 50°C and 10 min at 95°C and then subjected to 40 cycles of denaturation at 95°C for 15 s and annealing and elongation at 60°C for 1 min. PCR data were acquired from the Sequence Detector Software (SDS version 2.2, Applied Biosystems) and quantified by the relative standard curve method using serial dilutions of pooled cDNA.

Efficiency curves were generated using serial dilutions of cDNA in abscissa and the corresponding cycle threshold in ordinate.

Efficient partition of hybrid meshes has been accomplished by transforming them to suitable graphs and using serial graph partitioning algorithms.

Exact quantification was achieved by serial dilution with cDNA produced from total RNA extracts using serial dilution steps.

Using serial section, intra-hepatic PD1 and PD-L1 expression was also detected dynamically by immunohistology.