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Earlier diagnosis by using serial Aspergillus galactomannan antigen test in the modern medical era to detect IFS, may lead to early introduce anti-fungal agent and surgical debridement, and potentially decreased morbidity and mortality in high risk patients.
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All compounds were evaluated for in vitro antibacterial activities against Escherichia coli and Staphylococcus aureus strains and in vitro antifungal activity against Candida albicans and Aspergillus niger strains by using serial dilution method.
Positive samples were quantified by using serial dilutions.
These values were determined by using serial sections with Hoechst nucleus staining.
A standard curve was generated by using serial dilutions of CaCO3.
The synthesized compounds were evaluated for their in vitro antimicrobial activity against Gram-positive bacteria (Staphylococcus aureus and Streptococcus pyogenes), Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) and fungi (Candida albicans, Aspergillus niger, and Aspergillus clavatus) using serial broth dilution method.
Numbers of viable bacteria were estimated by limiting dilution using serial ten-fold dilutions of samples.
All assays were performed in duplicate and by the standard curve method using serial cDNA dilution.
Efficient partition of hybrid meshes has been accomplished by transforming them to suitable graphs and using serial graph partitioning algorithms.
Exact quantification was achieved by serial dilution with cDNA produced from total RNA extracts using serial dilution steps.
A set of LAMP primers that amplify the ribosomal DNA of the large subunit of Aspergillus fumigatus, Penicillium expansum, Penicillium marneffei, and Histoplasma capsulatum was designed, and the LAMP reaction was performed using serial concentrations of these fungal genomic DNAs as templates in the presence and absence of PPase.
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