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These libraries were screened by using sera from breast cancer patients.
In a pivotal study, Talal and colleagues [ 7] performed immunoblotting against HIV-1 proteins by using sera from 47 HIV-1-seronegative patients with primary SS.
The present findings were revealed by using sera from both lSSc and dSSc patients who were not under treatment with immunosuppressant or disease-modifying drugs.
Thus, the DSN was calculated by using sera from a cohort of anthrax vaccine recipients who had received a minimum of four subcutaneous injections of AVA.
The CI-ELISA effectively demonstrated the specificity of the ELISA format by using sera from AVA vaccinees and clinically confirmed anthrax cases (8).
Therefore, we determined the percentage of positive MAT results for leptospirosis for each seropositive state by using sera from dogs with suspected clinical leptospirosis that were submitted to a nationwide veterinary diagnostic laboratory.
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Anti-PUF60 antibodies were assayed by ELISA using sera from patients with primary Sjögren's syndrome (SS; n=84), systemic lupus erythematosus (SLE; n=71), DM (n=267), polymyositis (n=45), inclusion body myositis (n=45) and healthy controls (n=38).PUF60 was identified as a new autoantigen.
In addition, a number of IVIAT identified antigens (BA0314, BA0485, BA1952, BA2805, BA2748, BA3737, BA5427, pXO2-08 and pXO2-42) were also previously identified by Gat et al. using a bioinformatics screening strategy to select B. anthracis ORFs for use in an in vitro transcription/translation assay followed by immunoprecipitation using sera from B. anthracis-infected animals [37].
Detailed patient characteristics are presented in Tables 1 and 2. We quantified serum omentin-1 concentrations by ELISA using sera from patients with CD.
The antigenicity of clone product was analyzed by immunoprecipitation using sera from all 39 BD patients in parallel with normal controls and control sera from Lupus and Sjögren syndrome patients.
No differences in the allergen repertoire were detected by IgE-immunoblot stusing usera sera from 2 maize-allergic patients.
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