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Maximum-parsimony trees were generated by using sequences from each gene fragment.
Primer sequences for the fifteen selected genes were designed by using sequences from NCBI and PRIMER3 [ 26].
Blastx searches of the WGS contig library were then carried out by using sequences from abyX (upstream) and abyT (downstream) to identify flanking regions.
Because only regions with higher genetic diversity are most likely to be the virus source, we calculated the genetic diversity of 5 geographic regions by using sequences from 2006 through 2011 for which month of isolation was known.
Phylogenetic trees were constructed by using sequences from clinical isolates in the same cluster and related type strains as identified by the The All-Species Living Tree Project (release 102) (30 ) and/or List of Prokaryotic Names with Standing in Nomenclature (31 ).
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By using sequence from more than two related species and by making comparisons between evolutionary models within a likelihood framework, it is possible to identify both lineage-specific trends and to quantify the relative strengths of positive selection, negative selection, and neutral evolution (Yang 2007).
A phylogenetic study was performed by using sequences obtained from the two loci.
We were able to discern the genome of origin for the lichen sequences by using sequences derived from axenically cultured symbiotic partners as training sequences.
To date, nearly all analyses have been performed by using sequences obtained from viruses isolated during the first wave of infection; changes associated with viruses isolated during the second wave are largely unknown (6 ).
We have developed oligomer microarrays by using sequences derived mainly from the ESTs and cDNA sequences presented here, and we have successfully elucidated the characteristics of changes in gene expression in pig subcutaneous preadipocytes [ 51].
A broad environmental sampling from worldwide geographical locations coupled with experimental biochemical validation and comparative genomic analysis allowed us to test metabolic and evolutionary hypotheses difficult to approach by using sequence data from only a few environments.
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