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In both applications, by using sequence probes that cover all k-mers, a comprehensive picture of the effect of all possible short sequences on gene regulation is obtained.
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The present study compared gene coverage by using sequence annotations equivalent to public accession numbers available for 29,195 probes.
Genotyping for G6PD deficiency was performed by screening human DNA for single nucleotide polymorphisms in the G6PD gene (G202A) by a simple high throughput method using PCR followed by detection using sequence specific oligonucleotide probes (SSOPs) and ELISA based technology [ 33].
DNA samples were amplified by PCR and detection was performed using sequence specific DNA-probes.
HLA-B alleles were typed using sequence-specific oligonucleotide probes.
When the introns are small, discrimination between mRNA and closely related DNA and RNA sequences is still possible by using probes consisting of sequences in the neighboring exons.
As the genome of the Mediterranean mussel Mytilus galloprovincialis is not available at present, we have explored the possibility of reducing the whole genome sequencing efforts by using capture probes coupled with PCR amplification and high-throughput 454-sequencing to enrich selected genomic regions.
On the other hand, considering the small fraction (~0.5%) of the BAC libraries sampled, a more direct test of organelle contamination could be accomplished by using mitochondrial sequences as probes for hybridization.
To enable a direct comparison between microarray and sequencing data, both data sets were coupled by using the probe and sequence data.
Therefore, the detection of SNP in the random DNA sequence could be realized by using AIE probes and carbon nanotubes.
The authors concluded that by using probes targeting overlapping transcript sequence regions a greater level of concordance can be obtained compared to using UniGene ID or other sequence-matching approaches.
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