Protein partitioning assays were performed by using the selected system composition in previous works: Triton X-114 at 4% wt/wt, yeast fermentation supernatant at 60% wt/wt, McIlvaine buffer pH 7.00.
The high abundance of LRG1 peptides in the OvCa sample cohort, and near absence in controls, in conjunction with the previous identification of LRG1 protein in various diseases, suggest that future validation of LRG1 peptides by using quantitative selected reaction monitoring assays in larger numbers of urines is warranted.
The difficult problem of preventing lithium dendrite formation on charging was solved in these cells by using specially selected carbon powders as a base in which to insert lithium ions to form a weak compound that functions as a high-voltage, high-energy-density anode.
The surfactant alternated with gas (SAG) displacements are then conducted by using the selected CO2-philic surfactants dissolved in injection brine to study the interactions of foam and oil in porous media at the reservoir conditions.
The co-rotating TE22,6 mode is selected by using the in-house developed code GCOMS.
Potential respondents to the NHWS are recruited through an existing consumer panel selected by using opt-in emails, co-registration with panel partners, e-newsletter campaigns, and online banner placements.
The attitudes toward evolution questionnaire was created by the authors, in addition to using select items from previously existing scales designed to assess knowledge about evolutionary theory and resistance toward it (Shtulman 2006; Sikkink 2009).
However, for the present study we aimed at an approach that keeps the 3′-OH of the oligoribonucleotide available to retain the possibility for ligations to construct larger RNA, e.g., by using in vitro selected DNA ligation enzymes.
A reverse transcription polymerase chain reaction (RT-PCR) was performed on 400 randomly selected samples by using an in-house assay.
A total of 23 tick pools, representing 80 ticks, were manually homogenized, and RNA was extracted and tested for the presence of CCHFV RNA by using in-house assays that selected for 3 virus genes.
