Sentence examples for by using reverse transfection from inspiring English sources

By using reverse transfection microarrays we are able to transfect fluorescent reporter plasmids into 600 independent clusters of cells plated on a single microscope slide and image these clusters every 20 minutes.

siRNA-mediated knockdown of MITF was performed in two randomly chosen Motif 2 cell lines (NZM06, NZM15) by using reverse transfection with Lipofectamine RNAiMAX (Invitrogen) and a pre-designed siRNA targeting MITF (Ambion) according to the manufacturer's instructions, with a final siRNA concentration of 5 nM.

siRNA was transfected using reverse transfection with Hiperfect (Qiagen) according to the manufacturer's guidelines.

Transfection was performed using reverse transfection approach as described before.

Using reverse transfection and a robotic spotter, the first cell microarray for 2D cell culture was developed by the Sabatini group.

Transfection mixtures contained 50 nM siRNA and the complex was added to 106 cells seeded in T-25 bottles using reverse transfection.

(A ) High-content small interfering RNA (siRNA) cell-based screen using reverse transfection of the library in media containing serum for 72 hr, followed by 24 hr serum starvation, fixation and DAPI staining.

Using reverse transfection, miR-101 was overexpressed in human diploid fibroblasts, which resulted in a clear upregulation of miR-101.

Try using reverse psychology.

PANC-1 cells were transfected using a reverse transfection protocol.

Transfection of siRNA was achieved using INTERFERin® in vitro small interfering RNA (siRNA) transfection reagent (Polyplus) using a reverse transfection according to the manufacturer's protocol.