Sentence examples for by using reverse transcriptase from inspiring English sources

By using reverse transcriptase PCR (RT-PCR), 5′ and 3′ rapid amplification of cDNA ends (RACE) with designed, degenerate primers, a novel cDNA sequence was obtained.

Double-stranded cDNA was synthesized from the RNA fraction by using reverse transcriptase from avian myeloblastosis virus.

To determine the expression profile of FLG2, we first compared its expression with that of the FLG gene by using reverse transcriptase PCR (RT-PCR) analyses.

The eluted mRNA were reverse transcribed to cDNA by using reverse transcriptase (Sigma Aldrich, St Loius, MO) and oligo-dT primers (invitrogen) as per the instructions and PCR was performed with specific primers.

Mosquitoes were sorted by species and tested for WNV by using reverse transcriptase PCR assays.

Total RNA was reverse transcribed with random hexamers by using reverse transcriptase (Invitrogen, Carlsbad, CA, USA).

Interestingly, ER mRNA can be detected in both cell types by using reverse transcriptase-polymerase chain reaction (RT-PCR).

To the Editor: Stockton et al. have reported the detection of human metapneumovirus (HMPV) by using reverse transcriptase-polymerase chain reaction (PCR) in patients with influenzalike illness (1 ).

A SNAP_00018439 mRNA was detected by using reverse transcriptase-polymerase chain reaction (RT-PCR) and appears to encode the PsGEF protein containing only the C2 and PDZ domains.

Using the sequence information of codons corresponding to the presumed first and last amino acid residues of the Hydra CAD protein, the full-length coding region was cloned by using reverse transcriptase-polymerase chain reaction (RT-PCR).

We then investigated the putative changes in the downstream effector pathways by using reverse transcriptase-polymerase chain reaction (RT-PCR) and western blot analysis of the developing nasal region at 10.5 days post coitum (d.p.c).