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This assay was determined by using reverse phase C18 column (4.6 mm × 250 mm, 5 μ), while UV detector was set at a wavelength of about 215 nm.
The flavonoid compounds present in the polar solvents can be separated by using Reverse Phase HPLC equipped with a photo-diode array detector (RP-HPLC-PDA).
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Because neither compound was sufficiently retained by using reversed-phase chromatography, a column-switch LC system combining a reversed-phase and a Hypercarb column was developed.
The concentrations of glucose and lactic acid were determined by using reverse-phase high performance liquid chromatography (RP-HPLC) (Waters 2695, Separations Module and Waters 2410, Refractive Index Detector).
The analysis revealed that five peptide sequences have similarity with the stretches of secreted lipoprotein of Methylomicrobium album BG8 (Protein View: gi|381152247) and displayed 70% coverage with a significant homology (Figure 5). Figure 3 Separation of the antimicrobial lipopeptide from acidic methanol extract by using reversed-phase HPLC.
The acylglycerol composition of the extracted oil sample was determined by using reverse-phase high-performance liquid chromatography (RP-HPLC) (Alliance model Waters e2695 Separation Modules, UK) equipped with ELSD (Alliance model Waters 2424 ELS Detector, UK).
In addition, the Cancer Proteome Atlas (TCPA) provides ready access to cancer proteome data, which were generated using 3467 TCGA tumors and 439 cell line samples by using reverse-phase protein arrays [ 71].
The ascorbate content of the kiwifruit, plasma, urine, leukocytes, and muscle tissue was analyzed by using reverse-phase HPLC with a Synergi 4 micron Hydro-RP 80-A column (Phenomenex NZ Ltd) and an ESA coulochem II electrochemical detector (ESA Inc) as described previously (18).
Separation of complex peptide mixtures was achieved by using reversed-phase chromatography.
The purified samples were separated by using reversed-phase chromatography and analyzed by ESI-MS in fragmentation mode.
Its molecular structure (supplementary material Fig. S1) was identified by using mass spectrometry, and the purity of the compound reached 99%, as analyzed by using reversed-phase high-performance liquid chromatography (HPLC).
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