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The present study was designed to investigate the changes of PN-1 mRNA level and localization in the tracts during implantation and early pregnancy by using reverse transcription (RT -polymerase chain RT -polymerase and in situ hybridization.
The RNA was then analysed by using reverse transcription and quantitative PCR (qRT-PCR).
cDNA was synthesized by using Reverse Transcription System Set (Promega, Mannheim, Germany) following the manufacturer's instruction.
The synthesis of cDNA was performed by using Reverse Transcription Reagents (Promega, Madison, Wisconsin, USA).
A total of 26,746 swab specimens were screened by using reverse transcription PCR.
Nasopharyngeal aspirates were tested by using reverse transcription PCR, as previously reported (6 ).
Similar(22)
The gene expression of peroxisome-proliferator-activated receptor γ2 (PPAR-γ2), the adipocyte-specific transcriptional factor, was also investigated by using reverse transcription-polymerase chain reaction.
The most-definitive approach is nucleic acid detection, performed by using reverse transcription-polymerase chain reaction (RT-PCR) technology.
The levels of mRNA were quantitatively measured by using reverse transcription-polymerase chain reaction.
Steady-state levels of inducible nitric oxide synthase (iNOS) mRNA were determined by using reverse transcription-polymerase chain reaction analysis.
The presence of the receptor was confirmed by using reverse transcription-polymerase chain reaction (RT-PCR), functional assays of PGF2 alpha-stimulated inositol phosphate hydrolysis, and intracellular calcium measurements.
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