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The mutants were also assayed with regard to their ability to discriminate between sense and missense codons by using reporters described in the Supporting Information Materials and Methods S1.
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By using the Cre regulated RFP GFP reporter described in the paper, the authors can stably capture exceedingly rare expression from within a silent mating type cassette.
Responsiveness of human NOXA/PMAIP1 promoter −198/+45 region to Sall2 was studied using a reporter described previously.
However, it is present, as previously described using reporter mice and as revealed by in situ hybridization [21].
Reports submitted by consumers were identified using reporter field.
But yesterday Mr. Giuliani abandoned the word "decency," saying that reporters were trying to demean his commission by using it to describe the panel.
In keeping with this hypothesis, we confirmed the transcriptional repression of miR-24-2 promoter, upon IL-1β addition, by using a reporter luciferase assay previously described [ 28].
NF-κB reporter assays were performed as described previously by using a reporter plasmid NF-κB-Luc containing five tandem NF-κB binding sites upstream of a luciferase gene [18].
Alterations in gene expression regulated by TGF-β were assessed by using a reporter gene assay that monitored changes in luciferase expression driven by the synthetic SBE (Smad-binding element) promoter, as described previously [ 22].
Also, by using a reporter quantum dot, this nanobarcode platform can monitor and quantify gene expression level with improved speed and sensitivity.
The normalization efficiency was monitored by using chloramphenicol reporter gene.
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