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The technique platform was optimized and evaluated by using reference samples and artificial samples, which can specifically detect down to 3 copies of single or mixed plasmid templates.
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In addition, due to differences between primary cluster centers determined from reference samples and actual cluster centers determined from the test samples, CNs called by Gaussian mixture modeling using reference samples will also be misestimated.
These results show that the amount of SNP alleles can be accurately determined on the DNA level by Methods I and II using reference samples with the two SNP alleles present in known ratios.
The equipment was regularly calibrated using reference samples provided by the manufacturer.
Finally, the paper proposes a simplified method for estimating stress-strain relationships from the compressive strength and its corresponding strain of tested samples by using reference normalized curves.
The protein level in the diluted sample was calculated from a reference curve generated for a given assay by using reference standards containing known concentrations of appropriate protein.
One way is by using reference points.
Thus, using reference blood samples from blood donors is suitable.
Phasing and imputation Imputation is performed with different kinds of software and is a way to predict not genotyped variants, located between genotyped variants in haplotyped blocks, by using a reference sample where a greater number of variants are genotyped [96].
By adjusting using the reference sample, the biases were correctly compensated.
Though other inter-platform normalization methods and clustering methods were tested (z-score, quantile normalization), we found that only by using a common reference sample and transforming single intensity data into a log ratio enabled us to overcome the platform-dependent bias and observe cross-platform cell line-specific clusters.
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