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This study is an extension of Feder et al. 's (2013) FIT that allows for fluctuations in population size by using reference loci.
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In this study, we proposed a neutrality test, the FITR, to accommodate fluctuations in population size using reference loci.
One way is by using reference points.
As described in the next section, the FITR also uses neutral reference loci.
Stop using references.
We identified 2,613 variable loci in this analysis by using as reference genome the prototype pandemic V. parahaemolyticus clonal strain RIMD221633 (available from GenBank, www.ncbi.nlm.nih.gov/genome/ term=vibrioparahaemolyticus) (10 ).
Beside reference-free methods [ 22, 23], the majority of methods for whole blood are based on existing reference database of sorted blood cells by using differentially methylated loci across major leukocyte types [ 8, 14, 18].
Here, we expand upon the FIT by introducing a test that explicitly allows for changes in population size by using information from independent reference loci.
EEG data were recorded with a linked mastoid physical reference and were re-referenced by using an average reference.
These findings are validated by using the root-locus method from the discrete-control theory.
Percent on-target statistics (Table 1) were calculated by mapping reads to the reference loci using the Geneious native assembler (low sensitivity settings) and dividing the number of mapped reads by the total number of reads per each accession; the same method was used to determine read percentages for the chloroplast and mitochondrial genomes using the mitochondrial and chloroplast references.
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