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For that purpose, we have used an established method developed by Cawthon et al in 2002 [19], where, telomere length was determined by using real time q-PCR.
Measurement of telomere length by using real time PCR is an accepted method of choice from human blood samples as it requires small quantity of DNA.
Real Time PCR was performed by using Real Time TaqMan technology and analyzed on automated "StepOne System" (Applied Biosystem, Monza, Milan Italy).
Gene copy number was determined by using real time PCR based on the TaqMan fluorescence methodology.
The relative transcript levels of 16 differentially expressed unigenes were further validated by using real time PCR.
HPV DNA was amplified by using real time PCR with Mx4000 (Stratagene, La Jolla, CA, USA) (17 ).
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Image-guidance during ablation is achieved either by using real-time ultrasound (US) or magnetic resonance imaging (MRI).
£330m by using real-time information to pay tax credits.
Mobile robot localization consists in estimation of robot pose by using real-time measurements.
Therefore, the system can be more responsive to current VANET conditions by using real-time network monitoring.
Improving the efficiency of vehicle fleets by using real-time information is one of the first realizable steps.
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