Sentence examples for by using random primers from inspiring English sources

cDNA was synthesized from total RNA samples by using random primers (Invitrogen).

Usually, double-stranded DNA samples for a tiling array analysis are labeled by using random primers [26].

Four hundred nanograms of RNA (in triplicate) was reverse transcribed with SuperScript III by using random primers (Invitrogen, Paisley, UK).

cDNA was synthesized by using random primers (Invitrogen, Carlsbad, CA, USA) and M-MLV Reverse Transcriptase (Invitrogen).

Total RNA was then submitted to reverse transcription PCR with a High-Capacity cDNA Archive Kit (Applied Biosystems, Foster City, CA, USA) by using random primers according to the manufacturer's instructions.

Total RNA was isolated by using TRIzol reagent (Invitrogen Corporation), and RT was carried out by using random primers and Ready-To-Go You-Prime First-Strand Beads (GE Healthcare).

Total RNA was prepared from liver tissue by using Trizol method and 1 μg RNA was reverse-transcribed by using random primer hexamers.

Reverse transcription was performed by using random primer, and PCR was conducted by using primers ev22 and ev22 to amplify a 270-bp PCR product of the 5′ untranslated region (7 ).

First strand cDNAs were produced from 1 μg of total RNAs by using random primer 9 (New England BioLabs) and SuperScript® III Reverse Transcriptase (Invitrogen) following the supplier's instructions.

First strand cDNA was synthesized by reverse transcription reaction by using random primer mix, and used as a template to amplify genes of interest; for this specific primers against ANP (atrial natriuretic peptide), BNP (brain natriuretic peptide), collagen I, MMP-9 (matrix metalloproteinase-9) were designed.

Total RNA was isolated from cell lines, tumour cells in clinical samples, and from PBMCs using Trizol reagent (Invitrogen, Carlsbad, CA, USA), and was used as a template for the synthesis of complementary DNA by RT using random primers (Invitrogen).