Sentence examples for by using random mutagenesis from inspiring English sources

This study was designed to enhance l-serine production in a wild-type C. glutamicum SYPS-062 by using random mutagenesis, optimization of media, and metabolic engineering.

In this study we show that binding characteristics such as affinity can be improved for CBM generated from the CBM4-2 scaffold by using random mutagenesis in combination with phage display technology.

It should also be noted that highly active mutants of CYP102A1.1 (P450 BM3), which were obtained by directed evolution using random mutagenesis, towards non-natural substrates such as short-chain hydrocarbons (Peters et al. 2003), drugs (van Vugt-Lussenburg et al. 2007), and xenobiotics (Whitehouse et al. 2008) contained mutations that are not located in the active site.

Overall the approach of using random mutagenesis, followed by in vivo selection, selective screening, and then genetic sequencing was found to be very promising.

Alternatively, strains obtained by genetic modification or breeding can also be further improved using random mutagenesis.

The production of SK secreted from Streptococcus equi was enhanced using random mutagenesis.

Protein engineering using random mutagenesis was mentioned to result in improved production of isobutene.

However, using random mutagenesis is not possible to achieve 100% saturation (finding all essential genes).

In this study, we have scrutinized several of the most commonly used random mutagenesis techniques by critically evaluating popular error-prone polymerase chain reaction (PCR) protocols as well as hydroxylamine and a mutator Escherichia coli strain mutagenesis methods.

Generalized transduction with P22 was used for random mutagenesis.

Improvement kojic acid production ability of A. terreus by random mutagenesis using different exposure time to ultraviolet light (5 40 min) was then performed to obtain a suitable mutant of kojic acid production (designated as C5-10, 7.63 g/L).