Sentence examples for by using random hexamer from inspiring English sources
cDNA synthesis was performed by using Transcriptor First Strand cDNA Synthesis Kit (Roche) according with the user manual by using random hexamer primers.
In the first step total RNA was unspecifically transcribed into cDNA by using random hexamer primers.
cDNA was generated from the total RNA samples by using random hexamer (New England biolabs Inc, NEB) and M-MuLV reverse transcriptase (NEB) at 42°C for 1 h.
Total RNA was reverse transcribed by using random hexamer primers.
The cDNA sample was synthesized by using random hexamer primers.
These were used as first-strand cDNA templates synthesised by using random hexamer primer.
RNAs were reversed transcribed into cDNA by using random hexamers and Superscript II Plus RNase H− Reverse Transcriptase (Invitrogen).
cDNAs were generated from 1 µg total RNAs treated with DNase I by using random hexamers and Moloney murine leukemia virus reverse transcriptase (LTI).
Complementary DNA was synthesised by using random hexamers and oligo dT as oligonucleotide primer from 200 ng total RNA using the stratascript first-strand synthesis system (Stratagene) in a total volume of 50 µl as recommended by the manufacturer.
Approximately 3 µg of total RNA from each animal was reverse transcribed by using random hexamers and Omniscript RT kit (Qiagen Inc, CA, USA) as per the manufacturer's instructions.
