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Analysis of serum IGFBPs by using quantitative western ligand blotting revealed that, in rabbits, the IGFBP-2 concentrations were quantitatively most abundant among the serum IGFBPs that we measured (Fig. 2B).
To confirm the expression prolife of the molecules we observed in immuno- histochemistry studies, we measured the expression again by using quantitative western blotting or quantitative RT-PCR.
By using quantitative Western blot analysis, the effect of age on endogenous IGF2 levels was measured in the hippocampus of 15-month-old versus 7-month-old wild-type (WT) animals.
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These predictions of the model were confirmed by experimental data using quantitative western blot and mass spectrometry analysis.
The total and biotinylated surface proteins were detected using quantitative Western blots as described above.
Synaptophysin, AktT and AktPi protein expression were assessed using quantitative western blotting.
The low SFK expression in DP thymocytes has also been reported by Olszowy et al. [46], who used quantitative Western blot analysis to measure Lck and Fyn protein levels in thymocyte subsets.
By using quantitative PCR and western blot analysis, we also demonstrated that BMX is increasingly elevated in mTBI rats in a time-dependent manner.
Ectopic expression of TGFBI was quantified by using quantitative PCR and Western-blotting.
Array-based cell line classification was validated by using quantitative real-time PCR (qPCR), western blotting, and immunofluoresence on selected cell lines and genes.
The upregulation of RANTES was verified by using quantitative real-time PCR (qRT-PCR) and Western blot analysis.
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