Sentence examples for by using quantitative reverse from inspiring English sources
Previously, we found that microRNAs (miRNAs) were stably present in sputum and reliably measurable by using quantitative reverse transcriptase PCR (qRT-PCR) assay [14 16].
By using quantitative reverse transcription-polymerase chain reaction, we found that circulating miR-141 was significantly associated with stage IV colon cancer in a cohort of 102 plasma samples.
Female lambs born in late autumn 2011 or early winter 2012 were followed bimonthly to assess natural SBV primary infection by using quantitative reverse transcription PCR (RT-qPCR) and seroneutralization (SN).
DOI: http://dx.doi.org/10.7554/eLife.08942.004 Validation of the above data by using quantitative reverse transcriptase PCR (qRT-PCR) confirmed the reduction in transcript levels for genes located around the HO-induced DSB 240 min after HO induction.
By using quantitative reverse transcriptase PCR analysis, we first determine plasma expressions of five miRNAs in a training set of 32 patients with malignant SPNs, 33 subjects with benign SPNs, and 29 healthy smokers to define a panel of miRNAs that has high diagnostic efficiency for lung cancer.
In order to explore the biological relevance of ABCD1 in the major CD34+-derived immune cell types, we compared the basal ABCD1 mRNA levels with those of the other peroxisomal ABC transporters, ABCD2 and ABCD3 in healthy male adults (25 48 years of age) by using quantitative reverse transcription (qRT -coupled PCR.
To determine the activation state of the cell-signaling network in lapatinib-resistant tumor cells, we evaluated the expression of >150 protein/phosphoproteins representing mediators of key cell processes by using quantitative reverse-phase protein arrays (RPMAs) [ 29].
By measuring gene expression using quantitative reverse transcriptase PCR, this set of 59 genes was profiled in 579 patients, the largest NB patient series so far [ 80].
The light-mediated changes in gene expression were measured by quantification of mRNAs using quantitative reverse transcription polymerase chain reaction (qRT-PCR) (see the ESI † for details).
Our estimate of the duration of the infectious period (8.3 d, 95% CI: [5.8, 14.3]) is consistent with measures of viral shedding, obtained by Peiris et al. [31] using quantitative reverse transcriptase on sequential nasopharyngeal aspirates/throat and nose swabs (NPA/TNS), in which maximum virus excretion occurs around the tenth day of illness (compare also [24]).
Selected differentially expressed genes identified by the microarray results were then confirmed using quantitative reverse transcription PCR (qRT-PCR).
