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Furthermore, we studied the voltage-dependence fast and slow inactivation by using protocols which with pre-pulse for different time duration (10 ms for fast inactivation and 200 ms for slow inactivation) under the impact of BmK I (Fig. 4).
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Study began by using protocol 1 which was modified as protocol 2 in the second part of the study.
Then, the culture supernatant was collected and measured the BR level by using the protocol which has been reported by Dhouib et al (Turcanu et al, 1998).
Experiments that relied on laboratory animals were performed in strict accordance with the Institut Pasteur's regulations for animal care and use protocol, which was approved by the Animal experiment Committee of the Institut Pasteur (approval number n°03 49).
Measurements at baseline and follow-up were made by essentially the same group of trained investigators, and by using the same protocols, which allows performing combined analyses.
Unlike previously published results, mutations in either aak-2 or sir-2.1 fail to block lifespan extension by DR using our protocol, which robustly extends lifespan of wild type worms whilst reducing both food intake and reproduction.
Significant financial benefit without clinical risk has been shown [ 48] by using a protocol in which crystalloids are the primary agent of choice in haemodialysis-induced hypotension.
We next tried several other incubation media and finally were able to enrich the GnRH-immunoreactivecells population by using a differentiating protocol which consisted in incubating dissociated tertiary fetal NS for 5 days in a DMEM/F12 plus FGF-2 (20ng/mediaedia followed by a further 5 days incubation in a DMEM/F12 plus B27 (0.5%) media cells grown on poly-D-Ornithine treated coverslips).
The security validation of the proposed protocol has done by using BAN logic, which ensures that the protocol achieves mutual authentication and session key agreement property securely between the entities involved.
The least disruptive approach regarding communication overhead is provided by Hybrid Hierarchical protocols which use Reactive Routing for inter-cluster paths, however they suffer from a typical delay in on-demand solutions when retrieving paths.
*Columns1 4 describe detection of infection by various protocols: 1 = infection reported using protocol; 0 = no infection reported using protocol, e.g., the first line corresponds to the districts in which infection was detected only by the active surveillance of poultry.
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