Anthropometric measurements, including height, weight and blood pressure, were measured by trained staff using protocols from the NHANES III Anthropometric Procedures Manual (26), as described by Boyer et al. (24).
ELISA was performed by using the protocol from Cossaboom with a few modifications (1 ).
HuR binding to specific regions of caspase-2 mRNA was analyzed by crosslinking-RNP-IP assay by using a protocol from Niranjanakumari et al., with slight modifications as described previously.
DNA was extracted by using a protocol adapted from Lamour and Finley (27 ).
Catalase (CAT) activity was measured by using a protocol adapted from [ 29].
These isolates were additionally indistinguishable by PFGE at the FDIU Laboratory, Pullman, Washington by using standard protocols (25) and were indistinguishable from those of the clinic B outbreak.
RNA was quantified on the Nanodrop ND-1000 spectrophotometer (LabTech, East Sussex, UK), and reverse transcription was performed by using manufacturer's protocols from the Enhanced Avian RT First Strand cDNA synthesis kit, oligo dT 23 primer, and a total of 200 ng of RNA (Sigma Genosys, Cambridge, UK).
By using this protocol on material from cycling cells, we confirmed enrichment of the heterochromatin protein Swi6/HP1 at outer centromeric repeats, subtelomeric regions, and the mating-type locus, although we did not detect any significant centromeric signal of Sgo2, which is consistent with previous reports [11, 19, 20].
