Sentence examples for by using protocols described from inspiring English sources
Amyloid fibrils of Aβ (1 40) peptide were produced in 10 mM NaOH, pH 7.5 and room temperature after 3 days of incubation by using protocols described previously [56], while the fibrils of hen egg white lysozyme were formed in 20 mM glycine buffer at pH 2.2 and 57°C after 14 days of incubation [57], [58].
RNA was isolated from chondrocytes cultured in agarose by using protocols described in the QIAquick Spin gel extraction and RNeasy kits, as previously described (Qiagen, Crawley, West Sussex, UK) [ 20, 21].
Three-week-old Balb/C female mice were anesthetized, and their inguinal mammary glands were cleared of host epithelium by using protocols described by Smith et al. [ 18- 20] (three mice per condition; six cleared mammary fat pads).
RNA was isolated from chondrocytes cultured in agarose by using protocols described in the QIAquick Spin gel extraction and RNeasy kits, as previously described (Qiagen, West Sussex, UK) [ 28, 37].
DNA fragmentation was evaluated by using protocol described by McGahon et al. (1995) with modification.
Tumour size measurements obtained by breast MRI, using protocols described elsewhere (Bonelli et al, 2010), were made at baseline and at the 14th day of treatment and were independently reviewed by two experienced radiologists.
Microarray hybridization was performed using the Affymetrix GeneChip Yeast Genome S98 Array using protocols described by Affymetrix (Santa Clara, CA).
A set of physiological characteristics including catalase and oxidase tests and nitrate reduction were assessed using protocols described by Shieh et al. [ 17].
Total RNA for both NimbleGen microarray hybridization and real-time qPCR experiments was isolated using protocol described by Chang et al. [ 47].
The severity of the lesions was scored and examined by using protocols previously described [ 5] as seen in table 1.
