Even in the absence of genomic/EST resources, conserved miRNAs can be identified by expression analysis using probes designed to detect conserved miRNAs.
Forty-eight bursa samples collected from IBDV infected commercial broiler flocks in the US were analyzed by real-time RT-PCR using probes designed for two epitope regions of VP2 denominated minor peak 1 and peak B. It was observed that 23, 48 and 44 samples tested with the minor peak probes Del-E, STC and F15, respectively, had a lower melting temperature (Tm) than expected.
The coding polymorphism PON1 192 was genotyped using the Taqman real-time polymerase chain reaction (PCR) method as previously described (Holland et al. 2006) using probes custom-designed by Applied Biosystems, Inc. (Foster City, CA).
By using oligo DNA probes designed to recognize the sequence of the single-stranded segment, it is possible to carry out hybridization assay without performing heat denaturation after amplification.
Real-time PCR was performed using human FAM TaqMan MGB probes designed by assay-on-demand gene expression products (Applied): TGF- β1: Hs99999918_m1; connective tissue growth factor (CTGF): Hs00170014_m1: plasminogen activator inhibitor 1 (PAI1): Hs 00167155_m1.
70-mer oligonucleotides were selected from the complete genome sequence of B. pertussis strain Tohama I in collaboration with OPERON by using microarray probe design methodology as described by OPERON [ 30].
To obtain the missing 3'- end, we took advantage of the indexed genomic library [ 28], which we analyzed in two sequential hybridization steps by using a specific probe designed from sequence M07D05u.
All DNA and cDNA samples were tested by real time PCR by using designed primers and probes potentially targeting the 16S rRNA and the Brucella Cell Surface 31kDalton Protein (bcsp31) genes, which are highly conserved in 6 species of the genus Brucella[ 27- 29].
High-pressure 129Xe NMR experiments have been performed by using a newly designed probe.
By using an especially designed probe and standard gelatin gels, we have set up a method to assess gel strength, the cut resistance, simulating a teaspoon, as well as food consistency and chewiness, just in one experiment.
