Sentence examples for by using primers designed from inspiring English sources

These results were compared in parallel with other cloning procedures described previously, based on the analysis of other genes (16S rDNA and ITS1) and also by using primers designed for rpoD on sequences from gamma-proteobacteria.

All sequence was verified by classical dideoxy sequencing by using primers designed along the draft sequence.

PCR products were generated by using primers designed to include ends of the gene that was excised as well as flanking regions of the gene.

culicifacies species B using PCR as both the species were found to be identical, the relative expression level of AcNOS transcript in both P. vivax infected sibling species was analyzed by using primers designed against An.

RT-PCR was performed by using primers designed in a previous study (14 ).

All positive samples were subjected to PCR by using primers designed for the gltA and ompA genes (6 ).

This was confirmed by sequence comparison between ASDE and a 4 kb fragment amplified by PCR using primers designed from the 5′ and 3′ ends of ASDE.

The corresponding three genes for mutanases were cloned by PCR using primers designed from each N-terminal amino acid sequence.

The mitochondrial cytochrome b sequences of both species were amplified by PCR using primers designed from conserved regions.

Another mutanase-like gene from one strain was also cloned by PCR using primers designed from conserved amino acid sequences among known mutanases.

Two ALV-A and 4 ALV-B env sequences were obtained by PCR using primers designed to detect ALV-A and -B respectively.