Sentence examples for by using primer walking from inspiring English sources

Coding region sequencing was based on partial sequences of strain BeH22063 (GenBank accession nos. X73466 [S segment], AY380581 [M segment], JN801039 [L segment]) and completed where necessary by using primer walking.

We further sequenced the genome of NC-WGP93C strain by using primer walking, 3′ and 5′ rapid amplification of cDNA ends (RACE) methods (3, 6, 7 ).

All amplicons were then sequenced using primer walking.

Complete genome of the GBoV1 was acquired using primer walking approach as previously described [11].

Near full-length genome of the construct was sequenced using primer walking method described previously [6].

The longer products were sequenced using primer walking.

LsRXR encoding EST clones were selected and completely sequenced on both strands using primer walking.

Representative clones were retrieved, and entire inserts were sequenced using primer walking.

Clones sequenced by using the primer walking (PW) method are shown separately according to the number of primer walkings.

Other top-down SCRaMbLEd plasmids were initially analyzed using restriction digestion and subsequently analyzed using a primer walking sequencing method.

Plasmids pTRACA 20, 22 and 30 were sequenced by GATC biotech AG (Germany) using a primer walking strategy, while pTRACA18 was sequenced by the Wellcome Trust Sanger Centre (Cambridge, UK).