Sentence examples for by using primer software from inspiring English sources

Rank Mantel analyses were performed (16 ) by using PRIMER software (Primer-E, Ltd., Plymouth, UK).

The primers were designed using primer software.

The following forward and reverse primers were selected by using PRIMER EXPRESS software and were synthesized by TaKaRa Co. Ltd.

Primers for RT-qPCR were purchased from SuperArray for DHS quantitation or designed by using Primer 3 software.

Primers for pyrosequencing genotyping were designed by using primer design software from Biotage (Table S11 in Additional file 2).

Primers for SNP genotyping were designed by using primer design software from Sequenom (Table S11 in Additional file 2).

PCR primers were designed manually or by using Primer 3 software (http://frodo.wi.mit.edu/cgi-bin/primer3/primer3.cgi) so that the Tm values were between 55 and 60°C.

Consequently, we designed primers from closely related salmonids species by using Primer 3 software (Rozen and Skaletsky 2000) to obtain PCR products ranging from 90 to 150 bp.

Primers (Additional file 4: Table S1) were designed to anneal specifically to each targeted gene and three housekeeping genes β-Actin, β-Tublin and Ubc (ubiquitin C) of P. capsici and β-Actin of pepper [ 69] by using Primer 3.0 software for SYBR green real-time PCR (qRT-PCR).

The primer set was designed by using Primer Explorer V4 software and evaluated for its effectiveness in detecting Salmonella ser.

In-silico design was performed by using Primer Premier 5.0 software (Premier Biosoft, CA, USA).