Sentence examples for by using primer sets from inspiring English sources
It was observed that direct sequencing data of the hypervariable segment (HVS) 1 by using primer sets that generate an amplicon of 407 bp (long-primer sets) was different from results obtained by using newly designed primer sets that produce an amplicon of 120 139 bp (mini-primer sets).
By using primer sets OsLAP6-KO1 and OsLAP6-KO2, the CRISPR/Cas9 plasmids were generated and introduced into Agrobacterium tumefaciens strain EHA105, and transformation of Nipponbare was performed as described previously (Zou et al. 2017b).
For MSP, bisulfite-converted DNA was amplified by using primer sets specific for unmethylated and methylated promoter sequences in each candidate gene.
In addition, all reagents used in our analyses performed during 2012 2013 were subjected DNA amplification by using primer sets AB and CD (real-time and conventional PCR).
Phylogenetic analysis of NoV isolates was performed by using primer sets G1FF/G1SKR and G2FB/G2SKR for GI and GII, respectively, as described elsewhere (7 ).
Quantitative real-time polymerase chain reaction (qRT-PCR) was performed in triplicate by using primer sets and probes specific for TFF3 gene (Assay on Demand Hs00173625_m1, Applied Biosystems, Foster City, CA, USA).
Amplification of the joined genes after SOE [ 31] was performed by using primer set Sfi1L/LCNOT1.
Cloned DNA were sequenced by using primer set GLprimer2 : 5'-CTAGCAAATAGGCTGTCCC-3' and 5'-CTTTATGTTTTTGGCGTCTTCC-3'.
Real-time polymerase chain reaction was performed in duplicate by using primer set and probe specific for MGB-2 gene.
The 116 bp of housekeeping 16S rRNA gene fragment was amplified by using primer set of 16SF (5′-GAGAAGAAAGCCCCGGCTAA-3′) and 16SR (5′-ACCAGACTTAAAGAGCCGCC-3′) and treated as the internal control to verify that there were equal amounts of target cDNA in all samples.
This allele can be distinguished from other alleles by PCR using primer sets 'b', 'g' and 'f (as negative)' or by sequencing of the PCR-amplified fragments.
