Sentence examples for by using primer extension from inspiring English sources

The SNPs were genotyped by using primer extension of multiplex products with detection by matrix-assisted laser desorption ionization – time of flight mass spectroscopy using a MassARRAY Compact Analyzer (Sequenom, San Diego, CA, USA).

Several genes which exhibited higher transcript levels in the Δ rshA strain than in its parental WT strain in microarray analyses and/or in q-RT-PCR were chosen for promoter localization by TSP determination using primer extension analysis.

We performed high-throughput hydroxyl radical experiments by identifying cleavage positions using primer extension performed with fluorescently labeled primers, resolved by capillary electrophoresis [30], [31], [32].

We next determined the position of the transcription start site (TSS) of the mRNA produced by the reporter gene using primer extension with a 32P-labeled primer complementary to the luciferase gene.

The 2'-O-adducts were analyzed using primer extension.

For analysis of pgRNA encapsidation using primer extension (Fig. S2) we conducted reverse transcription primer extension as described [29].

The cassette is generated by PCR by using primers with circa 50 nt homology extension (H1 and H2).

We performed next-base extension studies by using primers containing different dNMPs opposite either dG or M1dG in the template strand.

In addition, the 932 bp of PCR products were generated by using primers (ldhAUDFCF1 and ldhAUDFCF2) that included homology extensions to the upstream and downstream regions and priming sequences for pTOP-FCF as a template (Table  5).

The D314A mutant of cytosine deaminase from E. coli was constructed by standard site directed mutagenesis methods using primer overlap extension from the pBAD322c-b0337 plasmid with the primers 5′-CGTCTGCTTTGGTCACGATGCTGTCTTCGATCCGTGGTATCC-3′ and 5′- GGATACCACGGATCGAAGACAGCATCGTGACCAAAGCAGACG-3′.

Multiplex PCR was used to simultaneously amplify DNA target regions of all five genes, and single stranded DNA (ssDNA) samples for microarray analysis were prepared by using a primer extension of amplicons in the presence of one primer of all genes.