Sentence examples for by using primer barcodes from inspiring English sources

PCR amplification of the ligated products was accomplished by using primer barcodes 1 12 (NEXTflex Small RNA Barcode Primers, BiooScientific) and DuroTaq 5x PCR Master Mix (BiooScientific).

A second slide made use of the multiplexing capability of the SOLiD system to sequence multiple samples at about 7.5 million sequences each by using primers having different barcodes.

The barcoding of the different libraries was finally achieved by PCR using primers which consisted of specific barcodes for each library.

The V3 V4 regions of bacterial 16S rDNA gene was amplified using primer set 338F (5′-ACTCCTACGGGAGGCAGCA-3′) and 806R (5′-GGACTACHVGGGTWTCTA-AT-3′), with overhang sequences as adaptors to link to the barcodes at the 5′ end for each primer.

Subsequently, 16S rRNA genes were amplified using the primers detailed in the supplementary material followed by a second round of PCR using primers with adapters and barcodes (see Additional file 1 for full description).

We have demonstrated that, by using standard barcoding primers on eDNA extracted from soil, we are able to broadly sample taxa from the soil biota.

In that case, barcoding power could be increased by using both barcoded forward and reverse PCR primers, but this would restrict the length of the sequenced regions, as each region would only be sequenced in one direction.

By using 15 different barcoded sequencing primers and splitting the Roche 454 sequencing plate into four segments using gaskets, up to 60 samples can be analysed in a single DNA sequencing experiment.

But it can be overcome by using a barcoding technique.

By using 12 barcoded forward primers (columns) and 8 barcoded reverse primers (rows), it is possible to create a uniquely barcoded amplicon for up to 96 clones.

Raw reads were assigned to samples using the unique primer barcodes and filtered by quality (50 bp rolling window PHRED cutoff of 20) using Qiime (version 1.6) (Caporaso et al., 2010).