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In our study, most fungal contamination could be prevented by using previously reported PCNB concentrations on the YEM base medium (P-YEM).
The obtained SiNM can be located in the desired position of the designed layout by using previously reported transfer printing techniques.
In our experiments, induction of hepatocyte-specific genes in MSC failed by using previously reported culture conditions [14], [8], [22].
Differentiation ability of BM-MSCs into classical mesenchymal lineage cells including adipocytes, osteoblasts, or chondrocytes was verified by using previously reported methods (Figure 2).
PPSB-PEE was prepared by using previously reported method [ 12].
TP53 gene mutations were screened for exons 5 8 by using previously reported primers and methods [ 15].
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Exons and flanking intron regions of GCK, including tissue specific variants of exon 1, were amplified by PCR using previously reported primers (exons 1a, 2, 3, 4) [13] or chosen by the Primer 3 program (exons 1b, 1c, 5, 6, 7, 8, 9 and 10) [10].
We pursue this valuable approach by first using previously reported NMR structural data to propose a membrane-bound orientation of the peptide.
Several overlapping fragments covering the entire mt genome were amplified by PCR using previously reported primers and cycling conditions [ 49].
The single exon of the KCNJ11 gene and the 39 exons of ABCC8 were amplified by PCR using previously reported primers [ 16].
The 40 coding exons and adjacent splicing junctions of CHD5 were amplified by PCR using previously reported primers and procedures [ 12].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com