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We determined the mutation frequencies of the Beijing and EAI genotype strains by using previously defined critical drug concentrations of 1 mg/L for INH, RIF, and MXF and 5 mg/L for AMK (11, 12 ) (for details, see the Technical Appendix).
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For our study, we used previously defined ecologic regions (e.g., areas defined by the distribution of flora, fauna, geomorphology, climate, and soils) (28 ).
Substance abuse was defined by using previously published methods (24 ).
For construction of defined double mutants, V. cholerae strains carrying a deletion mutation was further transformed with genomic DNA fragments of appropriate strains (carrying TnFGL3 inserthe2 in the presence of chitin by using previously described methods26,36.
Finally we give the integral representation of the boundary value problem by using the previously defined operator.
For group based comparisons pGDMs were divided into insulin sensitive (pGDM-IS) or insulin resistant subjects (pGDM-IR) by using the previously defined cut-off of SI = 2.8 × 10−4 min−1/(μU/ml) [ 23].
All OEQs were evaluated by two examiners, using a previously defined answering key.
Platelets were identified by particle size using a previously defined region that includes >95% of CD41-positive events and the mean fluorescent intensity of 10 000 events was recorded.
Instead of using the previously defined general hospital service areas by Klauss et al, this paper defines orthopedic hospital service areas (HSAOs) that use Swiss orthopedic discharge data from 2000 2002.
Using the previously defined threshold13 (shown by the dashed line), patients are classified as having low IPRES (light blue), high IPRES (dark blue) or heterogeneous IPRES (orange) scores.
Conventional CVD risk factors were defined using previously reported methods by us [ 15].
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