Co-IP was performed on leaf PM fraction by using the Pierce® Co-IP Kit (Thermo Sci). as per the manufacturer's instruction.
The samples were analyzed for their protein content by using the Pierce BCA-kit (Thermo Fisher Scientific, Waltham, MA, USA), and 15 µg protein per sample were separated by SDS-PAGE on a 4 20% TRIS-glycine gradient gel (Thermo Fisher Scientific).
Membranes were developed by using Supersignal (Pierce, Tattenhall, UK) and chemiluminescence was detected by use of a Fluor-S molecular imager (Bio-Rad, Hertfordshire, UK).
The M-hMPV protein was labeled by using Fluorescein-NHS (Pierce) and the preparation controlled for the absence of endotoxins.
Blots were developed by using chemiluminescent reagents (Pierce).
Immunoreactivity was detected by using chemiluminescence (Supersignal; Pierce).
The protein concentration of homogenates was measured by using BCA method (Pierce, Rockford, IL, USA).
The membranes were then washed in TBS-T buffer and incubated with secondary antibody coupled to horseradish peroxidase (1 2,000 in 5%% non-fat milk in TBS-T; Cell Signaling Technology) for 1 h at room temperature and visualized by using enhanced chemiluminescence (Pierce Biotechnology).
Use piercing eye contact.
Based on NIJ IV standard, ballistic test was executed on the three kinds of samples by using 0.30″ armor piercing projectile (AP).
